BMP-2에 의한 협부 지방 성체 줄기세포의 골형성

김창현; 표성운; 이일규; 박철헌

SITE LINK

BMP-2에 의한 협부 지방 성체 줄기세포의 골형성
Osteogenesis by BMP-2 in adult stem cell derived from buccal fat pad

대한구강악안면외과학회지 2008년 34권 4호 p.412 ~ 418

김창현, 표성운, 이일규, 박철헌,

소속 상세정보

김창현 ( Kim Chang-Hyun ) - 가톨릭대학교 의과대학 구강악안면외과학교실
표성운 ( Pyo Sung-Woon ) - 가톨릭대학교 의과대학 치과학교실 구강악안면외과
이일규 ( Lee Il-Kyu ) - 가톨릭대학교 대학원 통합의학전공
박철헌 ( Park Cheol-Hun ) - 가톨릭대학교 임상치과학대학원 임프란트학과

KMID : 0355620080340040412

Abstract

Bone morphogenetic proteins (BMPs) in combination with stem cells gain more significance for their use in bone tissue engineering. The mesenchymal stem cell can be differentiated into osteoblast by the treatment of BMP. The aim of this study is to characterize the osteogenic differentiation process of adult stem cells derived from buccal fat pad according to BMP-2 within culture media and decide the appropriate concentration of BMP-2 to facilitate osteogenesis. The authors procured the stem cell from buccal fat pad and analyzed for presence of stem cell by flow cytomety against CD-34, CD-105 and STRO-1. The buccal fat derived stem cells (BFDC) were treated by application of the different concentration with BMP-2 of 0, 10, 50, 100 and 200ng/ml, respectively. And their ability to differentiate into osteogenic pathway were checked by alkaline phosphatase(ALP) staining, Alizarin red staining and RT-PCR for osteocalcin(OC) gene expression at 7, 14 and 21day of culture. Flow cytometric analysis and biochemical assays demonstrated that BFDC might be a distinguished stem cells, and mineralization was accompanied in proportion to BMP-2 concentration. However, with 100ng/ml concentration of BMP-2, the BFDC demonstrated most efficent staining pattern of ALP and Alizarin red. The feasibilty of the osteogenic differentiation in the group of both 50ng/ml and 200ng/ml of BMP-2 showed similar activity and relatively weaker than that of 100ng/ml. These results suggest that the BMP-2 stimulate osteogenesis by BFDC effectively and that bone induction might be controlled through negative regulatory feedback in higher concentration.